epicentre RF91025 MasterAmp™ High Fideli

epicentre RF91025 MasterAmp™ High Fidelity RT-PCR Kit

产品简介：北京中北林格供应epicentre RF91025 MasterAmp™ High Fidelity RT-PCR Kit，中北林格是epicentre***，提供epicentre产品货号报价查询。其他包括lucigen***，cellscript***，Biosearch technologies***，epicentre***，illumina***，ICLLAB***，ImmunoReagents***。</p>

 

产品名称:MasterAmp™ High Fidelity RT-PCR Kit

货号:RF91025

规格:25 Rxns

存储温度:-20℃

品牌：lucigen / epicentre</p>

供应商：中北林格</p>

产地：us</p>

发货地：北京</p>

 

epicentre RF91025 MasterAmp™ High Fidelity RT-PCR Kit  产品描述

• Highly accurate RT-PCR amplification of RNA templates for cloning, sequencing, expression, and transcription analysis.
• All-purpose RT-PCR in which full-length amplification and high accuracy are essential.
• Multiplex RT-PCR.
• RNA模板的高精度RT-PCR扩增，可用于***，测序，表达和转录分析。
• 通用RT-PCR，其中全长扩增和高精度至关重要。
• 多重RT-PCR。

The MasterAmp™ High Fidelity RT-PCR Kit is ideal for synthesis of accurate double-stranded cDNA products that will be used for cloning, sequencing, expression, or transcription analysis. The kit uses MMLV-RT Plus, a reverse transcriptase with an unsurpassed accuracy and reliability, and MasterAmp TAQurate™ DNA Polymerase, a high-fidelity PCR enzyme mix (Fig. 1). The kit also includes an optimized 2X RT-PCR Buffer PreMix (containing dNTPs, MgCl2, and buffer), random and oligo(dT) primers, and the MasterAmp PCR Enhancer with betaine,* which can substantially improve the yield and specificity of RT-PCR amplification.

MasterAmp™高保真RT-PCR***盒非常适合合成***的双链cDNA产品，该产品将用于***，测序，表达或转录分析。该***盒使用MMLV-RT Plus（一种具有***的准确性和可靠性的逆转录酶）和MasterAmp TAQurate™DNA聚合酶（一种高保真度PCR酶混合物）（图1）。该***盒还包括优化的2X RT-PCR缓冲液PreMix（包含dNTP，MgCl 2和缓冲液），随机和oligo（dT）引物，以及具有甜菜碱的MasterAmp PCR增强剂*，可大大提高RT的产量和特异性。 -PCR扩增。

Benefits

• High-fidelity RT-PCR (i.e., low error rates).
• Amplification of full-length products from RNA templates up to ~6 kb (Fig. 2).
• Random and oligo(dT) primers included for first-strand cDNA synthesis.
• Unique PreMix reduces pipetting steps and chances of contamination.
• Uses MasterAmp PCR Enhancer for better amplification of high-GC or other difficult templates.
• 高保真RT-PCR（即低错误率）。
• 从RNA模板扩增全长产物，***大约6 kb（图2）。
• 包括随机和oligo（dT）引物，用于***链cDNA合成。
• 独特的PreMix减少了移液步骤和污染机会。
• 使用MasterAmp PCR增强剂可更好地扩***GC或其他困难的模板。

 

Figure 1. Different RT-PCR protocols that can be used with the MasterAmp™ High Fidelity RT-PCR Kit.

图1.可以与MasterAmp™高保真RT-PCR***盒一起使用的不同RT-PCR方案。

 

Figure 2. The MasterAmp™ High Fidelity RT-PCR Kit amplifies full-length products from RNA templates up to ~6 kb. RT-PCR products from Tobacco Mosaic Virus RNA with primers that amplify a 5.5-kb region of the viral RNA. Lane M, Kilobase ladder; lane 1, RT-PCR using the MasterAmp one-step protocol; lane 2, RT-PCR using MMLV-RT and Taq DNA polymerase with a standard two-step procedure; lane 3, RT-PCR using TthDNA polymerase.

图2. MasterAmp™高保真RT-PCR***盒可扩增RNA模板中的全长产物，***大约6 kb。***花叶病毒RNA的RT-PCR产物，带有引物，可扩增病毒RNA的5.5-kb区域。M线，千磅梯；泳道1，使用MasterAmp一步协议进行RT-PCR；泳道2，使用MMLV-RT和Taq DNA聚合酶的RT-PCR，采用标准的两步操作；泳道3，使用Tth DNA聚合酶的RT-PCR 。

 

Figure 3. Amplification of a variety of RNA templates using a one-step protocol with the MasterAmp™ High Fidelity RT-PCR Kit. Lane M, 100-bp ladder; lane 1, human lung β-actin; lane 2, human lung GAPDH; lane 3, region of TMV; lane 4, E. coli 16S rRNA; lane 5, human CGα; lane 6, human heart β-actin; lane 7, region of TMV.

图3.使用MasterAmp™高保真RT-PCR***盒一步一步扩增各种RNA模板。 M道，100 bp阶梯; 泳道1，人肺β-肌动蛋白；泳道2，人肺GAPDH；TMV区域3泳道；泳道4，  大肠***  16S rRNA；人CGα5泳道；泳道6，人的心脏β-肌动蛋白；TMV的第7车道。