epicentre NT0911-100 Nextera™ DNA Sample

epicentre NT0911-100 Nextera™ DNA Sample Prep Kit

产品简介：北京中北林格供应epicentre NT0911-100 Nextera™ DNA Sample Prep Kit ，中北林格是epicentre***，提供epicentre产品货号报价查询。其他包括lucigen***，cellscript***，Biosearch technologies***，epicentre***，illumina***，ICLLAB***，ImmunoReagents***。</p>

 

产品名称:Nextera™ DNA Sample Prep Kit (Roche Titanium-compatible)

货号:NT0911-100

规格:100 Rxns (2 x NT0911-50)

存储温度:-20℃

品牌：lucigen / epicentre</p>

供应商：中北林格</p>

产地：us</p>

发货地：北京</p

 

• 制备与IlluminaSolexa®下一代测序仪器兼容的DNA文库。
• Preparation of DNA libraries compatible with Illumina Solexa® next-generation sequencing instruments.
• 制备与Roche 454™GS FLX和GS FLX Titanium™下一代测序仪器兼容的DNA文库。
• Preparation of DNA libraries compatible with Roche 454™ GS FLX and GS FLX Titanium™ next-generation sequencing instruments.

Nextera™DNA样品制备***盒（与Illumina兼容）专门设计用于制备与Illumina Solexa GAI和GAII仪器兼容的***组DNA文库。Nextera技术利用  体外转座*在单管反应中同时片段化和标记DNA。与现有方法相比，Nextera文库制备方法具有明显的优势，例如雾化，超声处理或剪切（表1）。

The Nextera™ DNA Sample Prep Kit (Illumina-Compatible) is specifically designed to prepare genomic DNA libraries compatible with Illumina Solexa GAI and GAII instruments. Nextera technology employs in vitrotransposition* to simultaneously fragment and tag DNA in a single-tube reaction. The Nextera library preparation method offers significant advantages over existing methods, such as nebulization, sonication, or shearing (Table 1).

Nextera™DNA样品制备***盒（与Roche 454兼容）是专门设计用于制备与Roche 454 GS FLX和GS FLX Titanium仪器兼容的***组DNA文库。Nextera技术利用  体外  转座*在单管反应中同时片段化和标记DNA。与现有方法相比，Nextera文库制备方法具有明显的优势，例如雾化，超声处理或剪切（表1）。

Nextera™ DNA Sample Prep Kits (Roche 454-Compatible) are specifically designed to prepare genomic DNA libraries compatible with Roche 454 GS FLX and GS FLX Titanium instruments. Nextera technology employs in vitro transposition* to simultaneously fragment and tag DNA in a single-tube reaction. The Nextera library preparation method offers significant advantages over existing methods, such as nebulization, sonication, or shearing (Table 1).

• 仅需要50 ng的起始DNA。
• 在不到2小时的时间内准备好可测序的文库。
• 结合特定于平台的标签和可选的条形码（图1）。
• 在Illumina Solexa GAI和GAII仪器上验证。
• Validated on Illumina Solexa GAI and GAII instruments.
• 在Roche 454 GS FLX和GS FLX Titanium平台上验证。
• Validated on Roche 454 GS FLX and GS FLX Titanium platforms.

图1（单击放大）。生成用于IlluminaSolexa®平台的库。 将目标DNA片段化，并用Nextera™酶混合物标记，该酶混合物的末端带有测序引物位点（蓝色和橙色）。具有四引物反应的受限循环PCR将桥式PCR（bPCR）兼容适配器（紫色和粉红色）添加到核心测序库中。可以在下游bPCR适配器（粉红色）和核心测序库适配器（橙色）之间添加可选的条形码（三角形）。Illumina Solexa兼容文库需要其他测序引物：阅读1个引物（蓝色/***箭头）；阅读2底漆（橙色/***箭头）；和Index Read Primer（***/橙色箭头）。

Figure 1 (click to enlarge). Generation of libraries for Illumina Solexa® platforms. Target DNA is fragmented and tagged with Nextera™ Enzyme Mix containing ends appended with sequencing primer sites (blue and orange). Limited-cycle PCR with a four-primer reaction adds bridge PCR (bPCR) compatible adaptors (purple and pink) to the core sequencing library. Optional bar codes (triangle) can be added between the downstream bPCR adaptor (pink) and the core sequencing library adaptor (orange). Alternative sequencing primers are required for the Illumina Solexa-compatible libraries: Read 1 Primer (blue/gray arrow); Read 2 Primer (orange/gray arrow); and Index Read Primer (gray/orange arrow).
 

图2（单击放大）。生成用于Roche 454™平台的库。 用Nextera™酶混合物对***组DNA进行片段化和标记后，进行四引物有限循环PCR，添加了与Roche 454兼容的接头序列（蓝色和橙色）。可以在上游乳液PCR（emPCR）适配器（蓝色）和转座子末端（***）之间添加可选的条形码（三角形）。

Figure 1 (click to enlarge). Generation of libraries for Roche 454™ platforms. After fragmenting and tagging genomic DNA with Nextera™ Enzyme Mix, four-primer limited-cycle PCR is performed which adds Roche 454-compatible adaptor sequences (blue and orange). Optional bar codes (triangle) can be added between the upstream emulsion PCR (emPCR) adaptor (blue) and the transposon end (gray).

 

Catalog No.Size

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Nextera™ DNA Sample Prep Kit (Roche Titanium-Compatible)   NT091155 Reacti***   NT09112020 Reacti***   NT0911-5050 Reacti***   NT0911-9696 Reacti***

Contents: Nextera Enzyme Mix (Roche Ti-Compatible), 5X Nextera Reaction Buffer (HMW), 50X Nextera Primer Cocktail (Roche Ti-Compatible), 50X Nextera Adaptor 1 (Roche Ti-Compatible), Nextera Control DNA, and 2X Nextera PCR Buffer.

*Note: The Nextera™ PCR Enzyme (see below) must be purchased separately when using any of the Nextera™ DNA Sample Prep Kits.

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Nextera™ DNA Sample Prep Kit (Roche FLX-Compatible)*   FL091155 Reacti***   FL09112020 Reacti***

Contents: Nextera Enzyme Mix (Roche FLX-Compatible), 5X Nextera Reaction Buffer (HMW), 50X Nextera Primer Cocktail (Roche FLX-Compatible), 50X Nextera Adaptor 1 (Roche FLX-Compatible), Nextera Control DNA, and 2X Nextera PCR Buffer.

*Note: The Nextera™ PCR Enzyme (see below) must be purchased separately when using any of the Nextera™ DNA Sample Prep Kits.

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Nextera™ PCR Enzyme (required)   EM09112020 Reacti***   EM09115050 Reacti***   EM0911-9696 Reacti***

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Nextera™ Bar Codes (optional)   FLBC095012 Bar Codes (Roche FLX-Compatible)   NTBC095012 Bar Codes (Roche Titanium-Compatible)Note: The bar coding kits are optional. Each kit contains 12 bar codes, and each bar code can be used to prepare up to 50 bar-coded libraries.