应用领域
- 用于任何目的的非***性RNA探针的生产，包括原位杂交和印迹实验。
- Production of nonradioactive RNA probes for any purpose, including in situ hybridization and blotting experiments.
AmpliScribe™T7氨基烯丙基RNA转录***盒可高产率合成氨基烯丙基标记的RNA（AA-RNA）。该***盒将5-（3-氨基烯丙基）-UTP（AA-UTP）整合到RNA转录物中。由于将AA-UTP以与UTP相同的效率掺入RNA中，所以获得了高产率的AA-RNA。然后可以用胺反应性生物素化***或荧光染料处理AA-RNA产物，分别获得生物素标记的或荧光标记的RNA。已经优化了反应条件，以持续生成均一标记的AA-RNA，该AA-RNA与荧光染料或生物素化合物结合后可产生高信号强度。与在体外将荧光或生物素标记的NTP直接掺入RNA相比，这种非***性标记的RNA的制备方法更有效，更便宜。 转录。
The AmpliScribe™ T7 Aminoallyl-RNA Transcription Kit enables high-yield synthesis of aminoallyl-labeled RNA (AA-RNA). The kit incorporates 5-(3-aminoallyl)-UTP (AA-UTP) into the RNA transcript. Since AA-UTP is incorporated into RNA with the same efficiency as UTP, high yields of AA-RNA are obtained. The AA-RNA product can then be treated with amine-reactive biotinylation reagents or fluorescent dyes to obtain biotin-labeled or fluorescently labeled RNA, respectively. Reaction conditi*** h***e been optimized to c***istently generate uniformly labeled AA-RNA that, upon conjugation to a fluorescent dye or biotin compound, yields high signal intensity. This method for making nonradioactively labeled RNA is more efficient and less expensive than direct incorporation of fluorescent- or biotin-labeled NTPs into RNA during in vitro transcription.
注意：建议使用 TargetAmp™氨基烯丙基aRNA扩增***盒来制备标记的靶标，以与DNA微阵列杂交。
Note: TargetAmp™ Aminoallyl-aRNA Amplification Kits are recommended for making labeled target for hybridizing to DNA microarrays.
好处说明
- 该***盒使用EPICENTRE的AmpliScribe高产量体外转录技术来产生***高可能的氨基烯丙基RNA产量。
- The kit uses EPICENTRE's AmpliScribe high-yield in vitro transcription technology to generate the highest possible yield of aminoallyl-RNA.
- 优化了体外转录反应条件，以产生***佳水平的均匀标记的AA-RNA，以实现染料或生物素结合后的高信号强度。
- In vitro transcription reaction conditi*** are optimized to yield optimal levels of uniformly labeled AA-RNA for high signal intensity after dye or biotin conjugation.
- More efficient and less expensive than direct incorporation of fluorescent- or biotin-labeled NTP.
- 与直接掺入荧光或生物素标记的NTP相比，效率更高，成本更低。
- RNase Inhibitor included.
- 包括RNase***剂。
