l Cover and incubate 30 min at 37℃.
l Remove the cover and empty wells, Wash microwell strips according to step, Proceed immediately to the next step.
l Add 50ul Substrate A and Substrate B to each well。Incubate for 10 min at 37℃。
l The enzyme-substrate reaction is stopped by quickly pipetting 50ul of H2SO4. stop reagent into each well, including the blank wells, to completely and uniformly inactivate the enzyme. Results must be red immediately after the addition of H2SO4.
FOR INFORMATIONAL USE ONLY ▪ DO NOT USE FOR PERFORMING ASSAY ▪ REFER TO MOST CURRENT PACKAGE INSERT ACCOMPANYING TESTKIT犬C肽(C-Peptide)ELISA Kit 货号：HRE-6027