Amyloid beta-Protein Immunohistostain Kit (β淀粉样蛋白******染色***盒)
Storage condition: Keep at 2-10 degrees C.
Manufacturer: Wako Pure Chemical Industries, Ltd.
Cat. No. Pkg. Size
299-56701 50Tests
Summary
This product is for research use only. Do not administer it to human.
The plaque formation of amyloid b-protein
(Ab) in the brain is one of well-known symptoms
of Alzheimer's disease which are reported to cause neural death.
This kit allows distinctive stain between the plaques of Ab40
(1-40) and Ab42 (1-42) in a section to each
other.
Features:
(1) Distinctive histo-staining of Ab plaques
of Ab40 and Ab42
in Alzheimer's diseased brain tissues.
(2) A high sensitivity histo-immune detection of Ab
plaques in tissue secti*** with low background
Kit contents:
(1) Blocking Serum: 10 mL
(2) Anti Mouse IgG (H+L), Goat, Biotin Conjugated: 10 mL
(3) ABC Solution (Strept***idin-biotin-peroxidase complex): 10 mL
(4) Formic Acid (90%): 15 mL
(5) Anti Amyloid b-Protein (1-40), Monoclonal
(Clone No.: BA27): 7 mL
(6) Anti Amyloid b-Protein (1-42), Monoclonal
(Clone No.: BC05): 7 mL
(7) Trypsin, Crystalline: 50 mg
Principles:
The antigen in tissue section is sequentially reacted with the primary
antibody specific to either Ab40 or Ab42,
and biotinylated anti mouse IgG as the secondary antibody and ABC reagent
in this order, resulting in formation of an antige-antibodies-ABC complex.
By addition of hydrogen peroxide and DAB, a substrate and an acceptor of
peroxide, the peroxidase linking to the complex catalysis hydrogen peroxide
to form oxidized DAB brown precipitate in the limited area of the plaques
localizing in the tissue which can be observed by an optical microscope.
References:
(1) Iwatsubo, T., Odaka, A., Suzuki, N., Mizusawa, H., Nukina, N. and
Ihara, Y.: Neuron, 13, 45 (1994).
(2) Saido, TC., Iwatsubo, T., Mann, DMA., Ihara, Y., Shimada, H. and
Kawashima, S.: Neuron, 14, 457 (1995).
(3) Odaka, A., Ishibashi, Y., Kikuchi, T., Kitada, C. and Suzuki, N.,:
Biochemistry, 34, 10272 (1995).
(4) Fukumoto, H., Odaka, A., Suzuki, N., Shimada, H., Ihara, Y. and
Iwatsubo, T.: Am. J. Pathol., 148, 259 (1996).
(5) Fukumoto, H., Odaka, A., Suzuki, N. and Iwatsubo, T.: Neurodegeneration,
5, 13 (1996).