DNA Extractor (R) SP kit （***、血浆的DNA提取***盒）
Grade: for Genetic Research
Storage condition: Keep at 2-10 degrees C.
Manufacturer: Wako Pure Chemical Industries, Ltd.
(用于存在于***或血浆中DNA片断的提取)
Cat. No. Pkg. Size
296-60501 50Tests
Summary
This product is for research use only. Do not administer it to human.
DNA extraction from serum and pla***a
DNA Extractor SP Kit efficiently extracts DNA fragments contained in serum and pla***a. Based on the Sodium Iodide (NaI) method1, this kit enables the whole procedures done in a single micro-centrifuge tube without using hazardous phenol/chloroform.
Recently, tumor-specific genes h***e been amplified and detected in the serum and pla***a of patients with various diseases such as lung, breast and colon cancer. Many of these reference articles are currently being published2,3,4.
The kit is a powerful pretreatment reagent for the detection and analysis of target DNA because of its high quality and yield.
Features
High DNA yield (about 100%) from ***all amount of serum and pla***a
The DNA extraction procedure is based on a simple centrifugal separation using Sodium Iodide and alcohol, that results in less loss of DNA.
Safe operation
No phenol or chloroform required.
Minimum contamination: The entire extraction of DNA can be done in a single centrifuge tube
Complete removal of lipid derived from blood
Less variability in extraction from sample to sample
Kit Contents (50 reacti***)
Enzyme reaction solution 1 x 10 mL
Protein digestion solution 1 x 250 uL (uL: micro-L)
Sodium iodide solution 1 x 15 mL
Alcohol solution 1 x 30 mL
Washing Solution (A) 1 x 50 mL
Washing Solution (B) 1 x 50 mL
References
Ishizawa, M., Kobayashi, Y., Miyamura, T. and Matsuura, S.: Nucleic Acids Res., 22, 1774 (1994).
Sozzi, G., Musso, K., Ratcliffe, C., Goldstraw, P., Pierotti, M. A. and Pastorino, U.: Clin. Cancer Res., 5, 2689 (1999)
Silva, J. M., Dominguez, G., Garcia, J. M., Gonzalez, R., Villanueva M. J., N***arro, F., Provencio, M., San, Martin, S., Espana, P. and Bonilla, F.: Cancer Res., 59, 3251 (1999)
Shao, Z. M., Wu, J., Shen Z. Z. and Nguyen, M.: Clin. Cancer Res., 7, 2222 (2001)