Dietary Fiber Assay Kit （食物纤维测定***盒）
Grade: for Food Analysis
Storage condition: Keep at 2-10 degrees C.
Manufacturer: Wako Pure Chemical Industries, Ltd.
Cat. No. Pkg. Size
291-59701 100Tests
Thermostable alpha-Amylase Solution 1 vial x 20 mL
Protease Solution 1 vial x 20 mL
Amyloglucosidase Solution 1 vial x 20 mL
Diatomaceous Earth 1 vial x 100 g
Measurement Outline
Sample dissolved in MES/Tris buffer
Digestion with thermostable alpha-amylase
Digestion with protease and amyloglucosidase
for 30 minutes at pH 6.3 and 60 degrees C
Ethanol Precipitation
Le***e 1 hour
Filtration
Wash with ethanol and acetone
Dry overnight
Kjeldahl Protein Determination and Ash Determination
Total Dietary Fiber Determination
说明书单询。~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
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