Amersham Hybond-N+ 带正电荷尼龙膜 0.45um 30cmx3m RPN303B
带正电荷的尼龙膜与核酸高达600μg/cm2的结合能力。建议使用***性和非***性的化学发光法和chemifluorescence检测系统。
Amersham公司Hybond™- N +产品在传统的Southern杂交有着出色的灵敏度。核酸样品可能是固定的，通过紫外线照射或烘烤，虽然紫外线固定是***好的***大的重复性。 Hybond N +是不适合碱印迹和固定。
Amersham Hybond™-N+
- Positively charged nylon membrane with a binding capacity for nucleic acids up to 600 µg/cm2¾recommended for use with radioactive or nonradioactive chemiluminescence and chemifluorescence detection systems.
- Recommended for all high density membrane arrays.
Technical Information
Amersham Hybond™-N+ yields excellent sensitivity in conventional Southern blotting. Nucleic acid samples may be fixed by UV exposure or baking, although UV fixation is best for maximum reproducibility. Hybond-N+ is not suitable for Alkali blotting and fixation.
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Multiple reprobing is possible without loss of membrane integrity.
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QC tested in genomic Southern blots using GE Healthcare products.
- Special sizes ***ailable on request.
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Human genomic DNA (1, 0.5, 0.125, 0.06 and 0.03 µg loadings) digested with HindIII and transferred to Hybond-N+. Hybridizaton with A. N-ras, B. p53, and C. bcl-2 labeled DNA probes and detected using Gene Images™ random-prime labeling with CDP-Star™ detection.
Membranes should be stored at room temperature in a clean dry atmosphere away from excessive heat, light, and noxious fumes. To prevent contamination, handle membranes using gloves or blunt-ended forceps. Hybond-N+ is stable for up to 3 years. Membranes should be kept in the bags in which they are received. Performance is c***istent when stored under the recommended conditi***.
| TECHNICAL SPECIFICATI*** | |
| Pore size | 0.45 µm |
| Nucleic acid size required | > 50 bp |