【GDNF】重组人***胶质源***营养因子

产品报价

￥800/2μg ￥2080/10μg ￥74880/1mg

背景资料

GDNF promotes the survival and differentiation of dopaminergic neur*** in culture, and is able to prevent apoptosis of motor neur*** induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, h***e also been described. Mutati*** in this gene may be associated with Hirschsprung disease.
GDNF enhances survival and morphological differentiation of dopaminergic neur*** and increases their high-affinity dopamine uptake.

产品描述

人***胶质源***营养因子重组蛋白是在大肠***中重组表达的，是由两条非糖基化多肽链组成的同源二聚体。该重组蛋白包含有2 x 135个氨基酸，总分子量为30，360Da。该人***胶质源***营养因子重组蛋白***终由标准的色谱技术纯化出。

来源宿主

大肠***中重组表达

序列

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Pro-Asp-Lys.

溶解建议

提供的人***胶质源***营养因子 (GDNF)产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含人***胶质源***营养因子 (GDNF)的10mM柠檬酸钠和150mM N***溶液中经透析并冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到100μg/ml，后继实验过程中可根据实验要求进一步稀释。

保存建议

人***胶质源***营养因子 (GDNF)冻干粉末在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存。请避免反复冻融。