LED fluorescent microscope MF22
The microscope is an epi-fluorescence microscope designed for fluorescent and microbiology applicati***. It combines with MSHOT self-owned fluorescence illumination in LED module and modified trinocular Olympus CX22 biological microscope which adopts infinity-corrected objectives. The fluorescent microscope can present clear full-field, high contrast fluorescent field of view and bright field at the same time. Meanwhile, Abbe condenser can increase the visibility and contrast of the specimen.
Benefits of MF22 LED fluorescent microscope
- Blue and green two colors fluorescence excitation group in one unit. Wider w***elength choice.
- Trinocular head and C-mount adapter can support to connect with camera for photography.
- Excellent infinity optical system, outstanding resolution and definition.
- Infinity plan achromatic objectives in 4X, 10X, 40X and 100X
- The mildew-proof treatment (anti-fungus) help to protect the optical components
- The height limit function can effective ***oid direct contact and friction between the objective lens and the specimen slide.
Advantages of fluorescence illumiantion in LED module
- 50,000 hours long working life time.
- No warm up or cooling waiting, can operate immediately after starting up.
- LED cold light illuminator, power s***ing, no ultr***iolet prevention device is needed
- Wide range of high effective fluorescent w***ebands to be chosen for multifluorescence
- Modular design meets ergonomics principle, convenience to use
Application sample
Microbiology (especially 2 colors Blue-Green excitati***)
TB diagnostic ( (Immunofluorescence Assays, especially Royal Blue excitation)
Recommend Fluorescein Application
| Color |
Specification |
Suitable Fluorescein |
| UV |
330-380nm >400nm >420nm |
DAPI / Hoechst 33342&33258 / AMCA/AMCA-X / Alexa 350 |
| Blue |
460-490nm >500nm >510nm |
GFP / EGFP / FITC / Alexa 488 / Cy2@ / Fluo-4 / FluorX@ / Fluoro-Jade |
| Green |
510-550nm >570nm >590nm |
PI / EB / EH |
| Royal Blue |
420-480nm >500nm >510nm |
Special for TB Diagnostic |
Parameters :
| Optical system |
Infinity optical system |
| Eyepiece |
Wide field WF10X(Φ22mm) |
| Objectives |
Objectives (anti-fungus)
4x NA: 0.10 WD: 27.8 mm
10x NA: 0.25 WD: 8 mm
40x NA: 0.65 WD: 0.6 mm
100xOil NA: 1.25 WD: 0.13 mm |
| Eyepiece tube |
Trinocular, 3 ways light switcher, inclined 30° |
| LED Epi-fluorescence illuminator (Bright field/ fluorescence switchmode: pull lever） |
Exciter color: Blue |
| Central w***elength 475nm |
| Exciting filter, w***elength 460nm~490nm permeable |
| 45° dichroic mirror, w***elength over 500 permeable |
| Barrier Filter, w***elength over 510nm permeable |
| Exciter color: Green |
| Central w***elength 530nm |
| Exciting filter, w***elength 510nm~550nm permeable |
| 45° dichroic mirror, w***elength over 570 permeable |
| Barrier Filter, w***elength over 590nm permeable |
| Focus system |
Fine focus minimum distance: 2.5μm, Exquisite: 0.3,
Pre-focusing knob: coarse adjustment knob tension adjustable |
| Nosepiece |
Fixed quadruple objective nosepiece |
| Stage |
Wire tran***ission, size: 120mm × 132mm, moving range: X Axial 76mm × Y Axial 30mm, single specimen holder |
| Condenser |
Abbe condenser, NA: 1.25 (oil), built-in iris diaphragm aperture |
| Tran***itted illumination system |
6 V 20 W halogen bulb,
AC adapter: 00-120/220-240 V 50/60 Hz, 0.42/0.25A
Power c***umption: 18.5 W |
| Optical interface |
1XC-mount |
| Fuse |
250V 0.5A |
| Power line |
Standard three plug
|
| Optional Epi-Fluorescence Illumination Excitation Group |
| Excitation color |
LED lighting central w***elength |
Excitation filter |
Dichroic mirror |
Emission filter |
| Royal Blue |
450nm |
420-480nm |
>500nm |
>510nm |
| Optional Digital Cameras |
| Mode |
Main Specificati*** |
Features |
| MC20-N |
1.4MP color CCD camera, 2/3” *** ICX285AQ CCD |
High sensitivity, low noise, fast, comparative to cooled camera |
| MC20 |
1.4MP color CCD camera, 2/3"*** ICX285 CCD |
High sensitivity to weak fluorescence |
| MC50 |
5.0MP color CCD camera, 2/3" *** ICX282 Progressive Scan Interline CCD |
High resolution, cooling mode optional |
| MD50-T |
5.0MP color CMOS camera |
High resolution, sensitivity, fast frame rate, USB2.0& USB3.0 |
| MD30 |
3.0MP color CMOS camera |
High clarity, cost effective, stable |
| MD50 |
5.0MP color CMOS camera |
High resolution,cost effective, stable |
