PH荧光探针BCECF
BECEF被广泛地用于细胞内pH的测定。它的pKa值为6.97，接近生理PH值，它可以灵敏监测细胞内溶液PH的变化。细胞内PH值较低时BECEF的荧光很弱，随着PH的升高，荧光增强幅度很大。505 nm和439 nm通常是非常有用的用于比率测量的激发波长，一般会将490 nm和450 nm的滤光片加在激发光源的前面，比率法测量一般是在530 nm的相对荧光强度下进行的。BCECF acid是膜不通透型的，因此必须用微***方法直接进入细胞内。BCECF-AM是乙酰甲酯化的BCECF，它通过孵育就能进入细胞。
订购信息：
|
货号 |
产品名称 |
规格 |
|
51009 |
BCECF, AM ester, 1mg/mL in anhydrous DMSO |
1ml |
|
51010 |
BCECF free acid (2',7' -bis-(carboxyethyl)-5-(and-6)-carboxyfluorescein) |
1mg |
|
51011 |
BCECF, AM ester |
10x100ug |
|
51011-1 |
BCECF, AM ester |
20x50ug |
|
51012 |
BCECF, AM ester |
1mg |
|
51013 |
5-(and-6)-Carboxyfluorescein (5-(and-6)-FAM, mixed isomer) |
100mg |
|
51014 |
5(6)-CFDA (5-(and-6)-Carboxyfluorescein diacetate) |
100mg |
|
51015 |
5-(and-6)-carboxy-2',7'-dichlorofluorescein |
200mg |
|
51016 |
5-(&6)-carboxy-2',7'-dichlorofluorescein diacetate |
100mg |
|
51017 |
5-carboxy-2',7'-dichlorofluorescein (single isomer) |
100mg |
|
51018 |
5-CFDA (5-Carboxyfluorescein diacetate, single isomer) |
100mg |
|
51019 |
5-Carboxyfluorescein (5-FAM, single isomer) |
100mg |
|
51021 |
6-CFDA (6-Carboxyfluorescein diacetate, single isomer) |
100mg |
|
51022 |
Flubida-2 |
5mg |
|
51023 |
5-carboxy-2',7'-dichlorosulfonefluorescein |
10mg |
|
51024 |
Flubi-2 |
5mg |
|
90040 |
5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester |
25mg |
|
90041 |
5(6)-CFDA, SE (5-(and-6)-Carboxyfluorescein diacetate, succinimidyl ester) |
25mg |